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KK-Nano 2022 - abstrakt Andrzej Julian Kulik

Wystąpienie ustne Andrzej Julian Kulik (UN-Sr)

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Using Friction Force Microscopy to study Molecular Recognition

Andrzej Julian Kulik1, Maciej Dendzik2, Malgorzata Lekka3, Wieslaw Andrzej Nowak4

1 Ecole Polytechnique Fédérale de Lausanne, LBNI, Ecublens, 1015 Lausanne, Switzerland
2 Department of Applied Physics, KTH ROYAL Nstitute of Technology, Hannes Alfvéns väg, 11419 Stockholm, Sweden
3 Department of Biophysical Microstructures, IFJ PAN, Radzikowskiego 152, 31342 Krakow, Polska
4 Institute of Physics, Nicolas Copernicus University, Grudziacka 5, 87100 Torun, Polska

Classical pull-up AFM experiments are used to study interaction between proteins. The main drawback of the method is only 1% of successful events, since low coverage of the sample surface has to be used to prevent multiple interactions. The tedious elimination of hundreds of F-d curves without unbinding events asks for specialized software or lot of time.

Lateral (Friction Force) unbinding is not only permitting for faster pulling speeds but also allows for quick localization of binding sites directly on a friction image. However, the exact calibration of lateral force constant can be tricky and often requires specialized equipment.

Here, we present an easy and fast method for lateral force calibration (SMOK - Single Molecule Optimized (K)Calibration). The method is based on scanning a dextran-covered surface at an angle (22.5 degrees) with respect to the surface. The characteristic kink on force-distance curve (1 nN) corresponding to the single molecule unfolding, is seen on both: normal and lateral signals.

Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

AJ Kulik, M Lekka, K Lee, G Pyka-Fościak, W Nowak - Beilstein journal of nanotechnology, 2015

A single-molecule stretching method for lateral and normal AFM lever calibration

M Dendzik, A Kulik, F Benedetti, PE Marszalek, G. Dietler - Nanotechnology, 2013