Wystąpienie ustne Dariusz Witt (RMA-Sr)
IN-VIVO GENE EDITING BY NANOTRANSDUCERS
1 Prochimia surfaces sp. z o.o., Aleja Zwycięstwa 96/98/F8, 81-451 Gdynia, Polska
2 Department of Biology, University of Pisa, , SS12 Abetone e Brennero 5, 56127 Pisa, Italy
3 Department of Medicine, University of Pisa, , SS12 Abetone e Brennero 5, 56127 Pisa, Italy
Nanotechnology is increasingly used in the diagnosis or treatment of diseases. New and improved nanomaterials for biomedical applications were developed. Gold nanoparticles (AuNPs) was considered the most interesting nanomaterial due to its unique optical, electronic, sensory and biochemical properties. AuNPs are potentially used for medical imaging, drug delivery, and cancer therapy for the early detection, diagnosis and treatment of disease.
The I-GENE project is focused on the concept of gene editing, in which the nuclease activity of Cas9 is replaced by a nanotransducer (NT) based on gold nanoparticles (AuNPs). The activation is constrained by a number of conditions. I-GENE technology is based on a hybrid system including:
- The DNA recognition element (sensor) – a module responsible to guide the NT in the desired genomic location.
- The nanoparticle - AuNPs or gold nanorods AuNRs as a plasmonic nanoparticle covalently linked to the g-RNA/dCas9.
- The activator - The heat generated by the NT (AuNPs or AuNRs) will be used for a thermo-inducible double strand DNA break or cleavage. The accomplishment of that goal can be verified by two different design strategies (namely A and B).
The I-GENE Project is funded by Horizon 2020, Call identifier: H2020-FETOPEN-2018-2020.